Bone Marrow Transplantation Techniques in Mice
Posted by: admin | February 11, 2009 | 0 Comments
Bone marrow transplantation techniques in rodents are of particular interest to me since my time spent at the Fred Hutchinson Cancer Research Center as a research associate for almost two year where rodent and dog models are utilized for bone marrow transplantation studies. During the 1960s and 1970s, a team of scientists at the Center, led by Nobel Laureate Dr. E. Donnall Thomas and including Dr. Rainer Storb, worked to perfect the bone marrow transplant procedure. Transplantation research utilizing animal models continues at the Center today, thus affording me the opportunity to learn bone marrow transplantation techniques in rodents, including collection and delivery of bone marrow. One of the more interesting techniques I learned was a survivable collection of bone marrow from a mouse.
Because of recent discussion at our Institute and on list servers, it was especially exciting to see these techniques addressed in a review article in the January 2009 issue of Journal of American Association for Laboratory Animal Science. The review, titled Principles of Bone Marrow Transplantation (BMT): Providing Optimal Veterinary and Husbandry Care to Irradiated Mice in BMT Studies, addresses transplantation techniques in mice including advantages and disadvantages of various delivery routes. The authors also point out the negative effects of technical errors on the procedures. To prevent such errors, they recommend:
- that personnel performing bone marrow transplantation techniques receive proper training (this is a basic principle but one that is often taken for granted).
- that personnel demonstrate their proficiency by performing bone marrow transplant techniques with saline ‘inocula’ into naïve animals.
- that the institution consider:
- developing a for-fee injection service.
- teaching tail-vein injection techniques and recommending other. techniques to surgical personnel.
In addition, the review addresses basic principles of the bone marrow transplant procedure, transplant-related complications, the role of animal irradiators, specific husbandry and veterinary care needs of animals undergoing bone marrow transplant procedures, and the side effects of opportunistic infectious agents. Please find the abstract and the link to the article below.
Principles of Bone Marrow Transplantation (BMT): Providing Optimal Veterinary and Husbandry Care to Irradiated Mice in BMT Studies.
Written On: February 11th, 2009.
Bone marrow transplantation (BMT) is the treatment of choice for many leukemias, solid tumors, and metabolic diseases. The field of bone marrow research is highly dependent on in vivo experimentation, because in vitro techniques do not mimic these complicated in vivo systems. Therefore, understanding the medical and husbandry care needs of these transiently immunodeficient bone marrow recipient animals is crucial for researchers, veterinary and animal care personnel. Here we discuss the principles of bone marrow transplantation, mouse pathogens that can interfere with transplantation research, and important husbandry and veterinary practices for mice that may help to minimize unnecessary infections during the transplantation process. Whole-body irradiation is one of the most common tools for myeloablation of the recipient's bone marrow.